flobosg

According to the article metadata:

  "datePublished":"2026-07-16T08:18:37+09:00"

The Nintendo 64 Disk Drive (64DD) had the Randnet Browser.

Mac Micro

Mac Nano, just like iPods!

Yes, as supplementary information

Still part of the article.

Usually, journals require raw, unmodified data to be deposited as supplementary information.

I would be more worried if the blotted area was different (the dark blob)

Or if more than one blob is present (i.e. blobs at different molecular weights) for a supposedly selective and specific antibody that should show exactly one blob on the blot.

Now, if while preparing the images they needed to do some editorial choices

Editorial choices on raw scientific data are a big no-no.

From the article:

This image is supposed to demonstrate that the antibody being sold works as intended. (…) Antibodies are near-ubiquitous but notoriously fickle laboratory reagents in biomedical research. For many applications, it is absolutely crucial that the antibodies that you use are selective (i.e., the antibody binds strongly to the target protein) and specific (i.e., the antibody binds to the protein of interest and little else).

Antibodies showing a different picture (Western blot) than what is expected can drastically change the interpretation of the results as well as the conclusion of a study, for example. It may also encourage scientific fraud by authors by forcing them to unknowingly/coincidentally make to a blot image the same (or similar) fraudulent modifications performed by the vendor.

Now I’m curious about how much of the blot photoshopping present in retracted papers can be attributed to these misleading verification data.

Same with the TIM barrel fold. It can catalyze a wide range of reactions.

since the data used to train AlphaFold is limited to existing experimentally determined protein structures

Protein sequences, but the point still stands.

8G ought to be enough for anybody.

A small nitpick: chlorophyll is the pigment, photosystems are the protein complexes containing it.

So it works. But there has got to be a simpler/better/non-toxic way to create an ion trap using proteins and make plants work better ...

I remember reading about designed minimal photosystem-like systems. I cannot find the actual paper now, though.

that structure is as/more important than sequence?

Structure is determined by sequence, so they are equally important. Structure is more conserved than sequence, mainly due to the physicochemical constraints that govern protein folding.

that "reaction centers" are what matter, and the rest is just "protection"?

Sometimes not even protection. Many enzymes can have plenty of its sequence/structure removed and still be functional. Natural proteins carry lots of evolutionary cruft.

What do you mean by "reaction center" - surely not physically central within the folded structure

I think they borrowed the term from photosystems/photosynthesis. But, to be more precise, what they actually meant is the active site of an enzyme; the location where the catalyzed reaction takes place.

(isn't it the surface shape that determines reactivity) ?

Shape is not enough, the chemical nature of the amino acid residues involved is also important. A single mutation in a key catalytic residue will shut down the enzyme even if the shape stays the same.

My PhD thesis addressed a similar question. I did a survey of sub-domain sized fragments shared between different protein folds. It turns out that there are plenty, even among folds considered evolutionarily distant.

This reminds me of structural studies in proteins encoded by de novo genes in eukaryotes. They are usually either intrinsically disordered or adopt a molten-globule-like state.

Natural selection has no analogy with any aspect of human behavior, However, if one wanted to play with a comparision, one would have to say natural selection does not work as an engineer works. It works like a tinkerer - a tinkerer who does not know exactly what he is going to produce but uses whatever he finds around him whether it be pieces of string, fragments or wood, or old cardboards; in short it works like a tinkerer who uses everything at his disposal to produce some kind of workable object.

―François Jacob, “Evolution and Tinkering” (https://web.mit.edu/~tkonkle/www/BrainEvolution/Meeting9/Jac...)

Not sure if I understand your reply. Currently, the Sequoia beta channel is only getting security patches.

switch to the Sequoia public beta channel!

https://news.ycombinator.com/item?id=47198977#47202707

One of the things that helped me is Brick (https://getbrick.com/), which blocks and unblocks apps using a small device with an NFC tag.

I was going to say the same! You can also check the recipe card here: https://www.cookingforengineers.com/recipe/19/Erics-Chocolat...

He’s talking about the past (“For a while, …”). Up to early 2010s, I would say.

A quick search gave me for example this one: https://genome.cshlp.org/content/26/1/36

I'm not familiar with Margaret Oakley Dayhoff

Then you’re one of today’s lucky 10,000. Any time!

Or rather Margaret: https://en.wikipedia.org/wiki/Margaret_Oakley_Dayhoff

The Red Bull Music Academy web series Diggin’ In The Carts has an episode featuring Koshiro: https://www.redbull.tv/en/page/page:rrn:content:episode-vide...

My favorite antibiotic is fire.

“No, John. You are the goblins.”

(https://doom.fandom.com/wiki/Repercussions_of_Evil#The_Story...)

If you liked David Goodsell’s illustration you can find more of his work at https://ccsb.scripps.edu/goodsell/. I’m a huge fan.

How Perl Saved the Human Genome Project: https://bioperl.org/articles/How_Perl_saved_human_genome.htm...

Yes, he is! (Which explains other of his mangas, Akagi):

He has been playing mahjong since junior high school days, and admitted that though he has rarely lost a game when he was in school, his current level of ability is average. According to him, he has "tournament luck" and has even won mahjong tournaments between mahjong manga artists. He has also participated in professional mahjong matches. He played about two games against Akagi and Kaiji's voice actor Masato Hagiwara, who is known as one of the best mahjong players in the entertainment industry, and made Hagiwara say "I don't think I can beat him."

―https://en.wikipedia.org/wiki/Nobuyuki_Fukumoto

In the Kaiji manga, the “Minefield Mahjong” arc uses a variation of Japanese Mahjong. It can be read without knowing the general rules (as I did), but I guess they make some of the scenes more understandable and/or impactful. Maybe I’ll give it a reread after checking this out.