abcc8

When I did the same, also 15 years ago, the lab on campus would only Sanger sequence my insert - and for targets larger than ~600bp I also had to include internal sequencing primers (that had to be ordered separately, shipped, etc). Plasmidsaurus will return the entire plasmid sequence so you can verify it is intact, you don't have to provide any internal sequencing primers, simple repeats will be more accurately sequenced, and you can just pop some tubes in a padded mailer in the post at room temperature.

I'm no longer doing wet bench work but Plasmidsaurus seems like a service I'd happily use.

I didn't read the entire document in depth, but perhaps it has been shown elsewhere that first year GPA is a good quality predictor of GPA in subsequent years.

Were your heater and cooler equally efficient?

The article is less about that but more about the need for clear policies and consistent adherence to said policies regarding plus sized passengers.

Thank you for sharing. This was one of the first funny videos I downloaded from the internet.

Yes and no. I enjoy getting personal tasks done in the morning before work, especially those that require a sharp mind. Like you mentioned, I feel worn out mentally and the end of the day, though I find I'm happier and more productive overall if I save a few sharp-minded hours in the early morning for myself.

People choose to be tested for Huntington disease, including many young adults who have recently turned 18 (in the USA). While, like yourself, I don't know what I would personally chose to do if faced with this situation, the availability of a specific test with a high NPV and low FP rate would be beneficial to have.

The only solution I can think of is for myself to buy a lifted truck or other similar unnecessarily large vehicle. I haven't done this, of course, but if I did I'd then be part of the problem but at least my eye line would be above that of the majority of other drivers. We have a sedan and a small SUV - I don't drive the sedan at night ever because of the headlight problem. My eyes hurt just thinking about this.

Mammalian cells in research labs are grown in media that is composed of 1) multiple powdered components that are readily obtainable through isolation from bacteria/yeast or synthesis and, 2) 10% FBS (fetal bovine serum). Until these cultured meat companies can come up with a suitable replacement for FBS, due to environmental, moral, cost, etc concerns, none of these solutions are terribly viable. I personally wouldn't want to eat (or pay for) a tiger streak that required liters of FBS to grow.

I'm wondering how they plan to get decent content. I just searched two of my interests, climbing and canoeing, and only a handful of uninteresting videos popped up. Are there specific topics that have engaging communities on the site?

The media is primarily reporting good news instead of actual news. Twitter has been the best source of images, videos, and descriptions of what is happening on the ground. Search #deprem (earthquake in Turkish) to see for yourself.

This type of data is being collected at large academic institutions who are doing longitudinal analyses of their patient population.

Exacrly, they would say the test detected a pathogenic variant in the SHH gene.

I would prefer unprocessed food as well but it sounds like cost is a major driver in this case.

Yes, the extra chromosome 21 comes from the mother in approximately 90% of cases.

I think so. I was getting skin irritation if I exercised while wearing earbuds and the AfterShockz eliminate that problem for me. Sound quality is good for podcasts. I don't listen to much music at all with them.

It is rather dishonest to compare a chromosomal disorder with obesity, don't you think? Trisomy 21 is a condition one is born with and has no control over. On the other hand it has been demonstrated time and time again that behavioral and diet changes can be used to control obesity.

Indeed. My haircut routine now includes my tragus, the tops of my ears, and giving my eyebrows a good trim. Oh the joy.

IMO, the content can often be unrelated to science, as many tweets even by established scientists are about politics or other topics. There is also a large contingent of early career scientists trying to use twitter to get their name out there. Having a strong twitter presence can help assistant professors when to comes time for their tenure review, as peer name recognition does play a part in the decision (but there are other, better, non-Twitter ways to do this, too).

Speaking from experience, while it may take a bit longer at the gas station to get filled up, you get quite used to it.

I think the comparison is (rightly) meant to highlight the different imaging capabilities of the two telescopes.

And mute their microphones.

Phase 1 trials are used to determine drug toxicity. All that reault really says is that the new drug won't itself kill the patients. It still needs to be compared relative to the standard of care in larger phase 2 and 3 trials.

They could be referring to a toilet paper roller. Just had to replace one of mine and realized while shopping that I haven't seen a proper metal one of those in in years.

At least tips won't be required for these self-driving deliveries.

The idea of learning to read silently and not though sounding out the words seems asinine to me. I couldn't stomach the entire article but it is difficult to imagine a rational argument for this approach.

Completely agree. Also, I believe that articles, tools, etc reaching the front page will diversify as these initial tools are built upon and taken different directions.

I would argue that a miracle anti aging drug would work well for the majority of the population, much like NSAIDs work well for the majority of the population. Bonus points for being cheap and easy to synthesize.

Such a great channel. Love the videos where he cooks on his wood burning stove in the mountains.

It isn't the most diverse cohort so a body such as the WHO might or might not choose to make recommendations based on this single finding.